Fig. 7

Downregulation of neurotoxic responses in gp120-stimulated astrocytes by targeting the JAK2/STAT3 signaling pathway. A Immunoblots showing the protein levels of JAK2/p-JAK2/STAT3/p-STAT3 in stimulated astrocytes (F_{6, 70} = 13.95, P < 0.0001; F_{6, 91} = 69.49, P < 0.0001, respectively). B The mRNA levels of Jak2 and Stat3. C Quantification of A1-specific gene in each treated group. D The mRNA levels of Il-1α, Il-1β, Il-6, Tnf-α in astrocytes. E The protein levels of cytokines in astrocyte culture supernatants as measured by ELISA. In A, E, data are presented as mean ± SEM. In B–D, values are shown in the box and whisker plot, where the line in the box corresponds to the median. P values are calculated using one-way ANOVA with Tukey’s multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001. compared to the control group. ^$P < 0.05, ^$$P < 0.01, ^$$$P < 0.001 compared to 150 pM HIV-1 gp120 treated group