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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: TREM2 deficiency inhibits microglial activation and aggravates demyelinating injury in neuromyelitis optica spectrum disorder

Fig. 5

Phagocytosis of microglia and degradation of myelin debris was attenuated after TREM2 deletion. A Representative images of Iba1, CD68 and dMBP staining in the striatum of WT and TREM2−/− mice at 7dpi. Scale bar, 5 μm. B Quantification of engulfed dMBP within CD68 per microglia in WT and TREM2−/− mice with NMOSD. N = 5 mice/group, n = 10 fields/group, Mann–Whitney U test. C Representative images of Oil Red O (ORO) staining (left), Iba1 and Bodipy staining (middle) and the corresponding 3D reconstructions (right) in the striatum of WT and TREM2−/− mice post-NMOSD induction. Scale bars, ORO staining images, 40 μm; fluorescent images, 10 μm; magnified 3D reconstruction images, 3 μm. D Quantification of the percentages of the volume of Bodipy+ staining within Iba1+ cells verse the volume of Iba1+ cells. N = 5 mice/group, n = 10 fields/group, two-way ANOVA with Bonferroni multiple comparisons. E Representative images of dMBP immunostaining and 3D surface rendering of confocal images showing volume reconstruction of microglia (gray), CD68 (green) and dMBP (red), as well as dMBP detected within microglial CD68+ structures at 28dpi in the striatum of WT and TREM2−/− mice. Scale bar, fluorescent images, 500 μm; confocal images, 10 μm. F Quantification of engulfed dMBP within CD68 per microglia at 28dpi in the striatum of WT and TREM2−/− mice. N = 5 mice/group, n = 10 fields/group, two-way ANOVA with Bonferroni multiple comparisons

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