Fig. 6

STAT3 is activated and co-localized with CXCL13 in ipsilateral SCDH of CPIP mice. A Western blot showing expression of p-STAT3 and STAT3 in sham (left) and CPIP model (right) mice on Day 0, 7 and 14. Upper panels indicated representative blot images and lower panel indicate pooled data. B Western blot showing expression of acetylated-STAT3 (Ace-Stat3). n = 4–6 mice/group. C–E Double immunostaining of p-STAT3 with NeuN (C), GFAP (D) and Iba-1 (E) in ipsilateral SCDH of CPIP mice. White arrows indicate p-STAT3 and NeuN co-labeled cells. F Double immunostaining of p-STAT3 with CXCL13 in ipsilateral SCDH of CPIP mice. White arrows indicate p-STAT3 and CXCL13 co-labeled cells. Quantification of cellular distribution was shown on the right (pooled from 4 to 5 mice/group). *p < 0.05, **p < 0.01 vs. Day 0. NS: no significance. Scale bar indicates 50 μm. One-way ANOVA followed by Tukey’s post hoc test was used for comparisons in A, B