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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: CXCL13 contributes to chronic pain of a mouse model of CRPS-I via CXCR5-mediated NF-κB activation and pro-inflammatory cytokine production in spinal cord dorsal horn

Fig. 9

Intrathecal CXCL13 injection induces CXCR5-dependent NF-κB activation in spinal cord and results in mechanical allodynia in naïve mice. A Western blot gels showing pNF-κB and NF-κB expressions upon intrathecal injection of recombinant CXCL13 (rCXCL13, 100 ng/10 μL) or vehicle (1% BSA). B Summary of Western blot data in A. C Western blot gels showing expression of pNF-κB, NF-κB in spinal cord tissues from Cxcr5−/− mice 24 h after CXCL13 or vehicle injection. D Summary of Western blot data in C. **p < 0.01. E Time points for intrathecal rCXCL13/Veh1 (1% BSA in PBS), PDTC/Veh2 (20% DMSO in PBS) injection, behavioral test and tissue collection in mice. F qPCR results showing Il6 gene expression in SCDH of Veh1 + Veh2, rCXCL13 + Veh2 and rCXCL13 + PDTC group of mice. G Representative photos showing GFAP staining in SCDH of 3 groups of mice. H Summarized GFAP staining intensity/field in SCDH of 3 groups of mice. I Representative photos showing c-Fos staining in SCDH of 3 groups of mice. Scale bar indicates 50 μm. J Summarized No. of c-Fos stained cells/field in SCDH of 3 groups of mice. K Time courses showing 50% PWT changes in Veh1 + Veh2, rCXCL13 + Veh2 or rCXCL13 + PDTC groups of mice. **p < 0.01 vs. Veh1 group. ##p < 0.01 vs. rCXCL13 + Veh2 group. L Summary of normalized AUC of curves in E. n = 4–8 mice/group. Two-way ANOVA with repeated measures followed by Tukey’s post hoc test was used for comparison in K. One-way ANOVA followed by Tukey’s post hoc test was used for comparison in F, H, J, L. Student’s t test was used for comparisons in B, D

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