Fig. 2

Inhibition of the glycolytic pathway abolished the activation of microglia induced by CKLF1. A Relative mRNA levels of inflammatory cytokines in microglia challenged by C27 with or without 2-DG (n = 8–10 per group). IL-1β: F (5, 54) = 15.51; IL-6: F (5, 48) = 29.78; TNF-α: F (5, 36) = 19.28. B Inhibiting of glycolysis blocked the phagocytosis induced by C27 in vitro. The amount of zymosan phagocytosed by microglia in each group was detected by imaging and flow cytometry, and the positive ratio of phagocytosis was quantified for each group (n = 3 per group). F (2, 6) = 15.43 C Relative mRNA levels of inflammatory cytokines in microglia challenged by C27 with or without galactose (n = 5 per group). IL-1β: F (5, 24) = 44.74; IL-6: F (5, 24) = 20.32; TNF-α: F (5, 24) = 19.1. D Inhibiting of glycolysis by galactose blocked the phagocytosis induced by C27 in vitro. The amount of zymosan phagocytosed by microglia in each group was detected by imaging and flow cytometry, and the positive ratio of phagocytosis was quantified for each group (n = 3 per group). F (2, 6) = 22.7. The data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control group