Fig. 5From: A breakdown of metabolic reprogramming in microglia induced by CKLF1 exacerbates immune tolerance in ischemic strokeGene expression in freshly isolated microglia from adult mice after acute or chronic stereotactic injection of C27. A Volcano plot showing changes in gene expression and upregulated genes in the microglia of C27 acutely stimulated mice that were significantly (p < 0.05) enriched for 20 BPs compared to PBS-treated mice. The importance of BPs is indicated by the intensity of red (n = 3 per group). B Volcano plots showing changes in gene expression and downregulated genes enriched in 20 significant (p < 0.05) BPs in the microglia of chronically C27-stimulated mice compared to acutely stimulated mice. The importance of the BPs is represented by the intensity of blue (n = 3 per group). C Volcano plot showing changes in gene expression the microglia of chronically C27-stimulated mice compared to PBS-treated mice (n = 3 per group). D Comparison of the significance of the top 20 BPs shared between the groups after acute and chronic treatment with C27 (n = 3 per group). E Heatmap depicting the transcriptional profile of selected BPs among the top 20 BPs (n = 3 per group). F Normalized expression of selected genes (n = 3 per group). IL-6: F (2, 6) = 18.14; TNF-α: F (2, 6) = 131.6; GLUT1: F (2, 6) = 117.1; PFKFB3: F (2, 6) = 18.82; TREM2: F (2, 6) = 13.66; CKLF1: F (2, 6) = 4.202. The data are presented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. vehicle group or acute groupBack to article page