Fig. 6

Chronic administration of C27 to the M1 cortex produced immune tolerance in vivo. A C27 was delivered into the brain at a dose of 10 μg through stereotactic injection once or four times, and the tissues were collected 24 h after the last injection. B The structure of microglia was revealed by Iba-1 staining, which was further analyzed by skeleton analysis in ImageJ. Scale bar = 10 μm. C Analysis of process lengths induced by C27 (n = 50 per group). F (2, 147) = 93.57. D Analysis of the number of endpoints per microglia induced by C27 (n = 50 per group). F (2, 147) = 1149. E Microglia could not phagocytose neutrophils after chronic administration of C27. Microglia and neutrophils were stained green and red, respectively, and the merged images showed neutrophils engulfed by microglia (n = 3 per group). Scale bar = 15 μm. F Lamp1 was decreased by chronic administration of C27 (n = 3 per group). F (2, 6) = 19.74. The data are presented as the mean ± SD. **p < 0.01, ***p < 0.001 vs. vehicle group