Fig. 3

Pregnancy induces genome-wide epigenetic and transcriptomic changes in CD4⁺ and CD8⁺ T cells. DNA and RNA extracted from CD4⁺ and CD8⁺ T cells from women with MS and healthy controls (HC) were analyzed during pregnancy by RNA-seq and Illumina Infinium DNA Methylation EPIC Array for DNA methylation. The Pearson correlation coefficients between gene counts (for RNA-seq) and the beta values of all detected CpGs (for methylation) for the comparisons 3rd-1st trimester and PP-3rd trimester for resting A CD4⁺ cells and B CD8⁺ cells in women with MS (upper panel) and HC (lower panel) are shown. Pearson’s correlation r is shown in the individual graphs for each comparison. All correlations had a p < 2.2 x 10⁻¹⁶. HC healthy controls, MS multiple sclerosis, PP post-partum