Fig. 2
From: Co-modulation of TNFR1 and TNFR2 in an animal model of multiple sclerosis
TNFR modulation does not affect immune cell phenotype. Hu/m TNFR1ki mice treated with either PBS (control) (A; n = 6), H398 (B; n = 8), EHD2-sc-mTNFR2 (C; n = 5), or H398 and EHD2-SC-mTNFR2 (D; n = 5) and were killed at day 20 of EAE. Spinal cord sections were immunolabelled with antibodies against both CD3 and FoxP3 to detect regulatory T cells using Vector SG (black) as a chromogen to detect CD3-positive cells and DAB (brown) to detect FoxP3-positive cells. E Quantification of double-labelled CD3/FoxP3-positive cells. For flow cytometry, splenocytes were isolated from mice treated with either H398 (n = 7), EHD2-sc-mTNFR2 (n = 4), H398 and EHD2-SC-mTNFR2 (n = 8) or PBS (n = 6) on day 20 of EAE. The percentage of F CD4-positive or G CD8-positive T cells co-expressing markers of either TH1 (IFNγ), TH17 (IL-17a), or Tregs (FoxP3) was assessed. Scale bar = 50 µm