Skip to main content
Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Co-modulation of TNFR1 and TNFR2 in an animal model of multiple sclerosis

Fig. 4

TNFR modulation is neuroprotective and promotes neuroprotective signalling pathways. Whole-mounted retinas were immunolabelled with an antibody against RBPMS to identify surviving RGCs for quantification. Representative images are shown from healthy hu/m TNFR1ki mice (A), or EAE mice treated with either PBS (control, B), H398 (C), EHD2-sc-mTNFR2 (D), or a combination of H398 and EHD2-sc-mTNFR2 (E). EAE retinas were extracted on day 20 of EAE and quantification performed (F; healthy, n = 8 retinas from 4 mice; control, n = 13 retinas from 7 mice; H398, n = 14 retinas from 8 mice; EHD2-sc-mTNFR2, n = 8 retinas from 5 mice; H398 and EHD2-sc-mTNFR2, n = 13 retinas from 7 mice). Western blotting was performed to determine the protein levels of G phosphorylated Akt (pAkt (ser 473), 62 kDa), Akt (62 kDa) and GAPDH (37 kDa), or J for phosphorylated NF-κB p65 subunit (pNF-κB (ser536), 65 kDa), NF-κB (65 kDa), and GAPDH (37 kDa) on unfractionated retinal lysates prepared on day 20 of EAE. Quantification (n = 4 per treatment group) was performed to assess the ratios of H Akt/GAPDH, I pAkt/Akt, K NF-κB/GAPDH, or L pNF-κB/NF-κB. Uncropped Western blots are shown in Additional file 2: Fig. S2. *p < 0.05, **p < 0.01, one-way ANOVA followed by Dunn’s multiple comparison test (F), one-way ANOVA followed by Dunnett’s multiple comparison test (H, I, K, L). Scale bar = 100 µm

Back to article page