Fig. 4
TNFR2 stimulation leads to an increased number of Tregs in the spinal cord at 18 dpi. Immunohistochemical analysis of FoxP3 staining of hu/m TNFR1-ki x hu/m TNFR2-ki mice immunized with MOG35-55 and treated either with saline, EHD2-scTNFR2 (TNFR2 agonist), ATROSIMAB (TNFR1 antagonist) or a combination (E + A). FoxP3 + cells (black) were counted in spinal cord sections of mice killed at 18 days post-immunization (dpi; n = 5/group; A and B) and at 25 dpi (n = 5–7/group; C and D). Data are presented as mean ± SEM and differences between groups were assessed with Mann–Whitney test. *p < 0.05