Fig. 4

Intracisternal injection of E protein upregulated GFAP in the cortex, hippocampus and olfactory bulb. A Representative images of GFAP expression in the cortex from mice receiving Vehicle or E protein injection. B Fluorescence area analysis showed that E protein significantly upregulated GFAP expression in the cortex. C Representative images of GFAP expression in the hippocampus (CA1, CA3 and DG regions) from mice receiving Vehicle or E protein injection. D–F Fluorescence area analysis showed that E protein significantly upregulated GFAP expression in the CA1, CA3 and DG regions of the hippocampus. G Representative images of GFAP expression in the olfactory bulb from mice receiving Vehicle or E protein injection. H Fluorescence area analysis showed that E protein significantly upregulated GFAP expression in the olfactory bulb. Scale bar: 50 μm. n ≥ 6 per group; *p < 0.05, **p < 0.01, ****p < 0.0001; Student’s t test