Fig. 2

β-arrestin1 is decreased in the reactive astrocytes of orthopedic surgery-treated mice. A mRNA levels of GPCR-related genes in the hippocampus were analyzed by RT-PCR. B Expression of β-arrestin1 and β-arrestin2 in the hippocampus of CON and POD mice. C Densitometric analysis of β-arrestin1 and β-arrestin2. D Representative immunofluorescent staining of GFAP (red) and β-arrestin1 (green) in hippocampal slices of CON and POD mice. E Representative immunofluorescent staining of Iba-1 (red) and β-arrestin1 (green) in hippocampal slices of CON and POD mice. F Relative co-localized signals of the GFAP-positive and β-arrestin1-positive immunofluorescent particles between CON and POD group. G Relative co-localized signals of the Iba-1-positive and β-arrestin1-positive immunofluorescent particles between CON and POD group. Data are analyzed by unpaired Student’s t-test. n.s means no significance. ^*P < 0.05 and ^**P < 0.01 vs. the CON group. Values are presented as means ± SEM from at least three independent experiments