Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium

Fig. 6

β-arrestin1-biased ligand induces the interaction of β-arrestin1 and Drp1 to inhibit mitochondrial fission in vitro. A Schematic of the experimental design. B Immunoblotting analysis of Drp1 proteins in cell lysates of primary astrocytes immunoprecipitated with β-arrestin1 antibody. C Immunoblotting analysis of β-arrestin1 proteins in cell lysates of primary astrocytes immunoprecipitated with Drp1 antibody. D Immunofluorescent staining of TOMM20 (green) and Drp1 (red) in primary astrocytes. E Relative co-localized signals of the TOMM20-positive and Drp1-positive immunofluorescent particles between groups. F Representative images of Mitotracker Green in primary astrocytes under confocal microscope. Data were analyzed by one-way ANOVA followed by Dunnet’s post-hoc test. *P < 0.05 vs. the CON group. #P < 0.05 vs. the LPS-MCM group. Values are presented as means ± SEM from three independent experiments

Back to article page