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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Role of macrophage autophagy in postoperative pain and inflammation in mice

Fig. 1

Confirmation of Atg5 gene knockout and autophagy deficiency in macrophages. A Polymerase chain reaction analysis of gene in peritoneal macrophages from wild type (WT), Atg5f/f, and Atg5f/fLysMCre (Atg5 cKO) mice. B Western blot analysis of ATG5 and SQSTM1/p62 proteins in peritoneal macrophages from Atg5f/f and Atg5f/fLysMCre mice (n = 5 each) cultured for 6 h under energy-depletion. C Densitometric quantification of western blot of ATG5, SQSTM1/p62, and LC3 proteins (n = 3–5 each). D Representative immunofluorescence images of macrophages. E LC3 immuno-staining density of macrophages (n = 3 each). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. cKO conditional knockout; WT wild type

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