Fig. 8
From: IL-10 production by granulocytes promotes Staphylococcus aureus craniotomy infection
IL-10 production by G-MDSCs is critical for inhibiting PMN bactericidal activity and TNF production. PMNs were exposed to live S. aureus USA300 LAC for 2 h at an MOI of 10:1 (bacteria:cell) in the presence of IL-10 KO or WT G-MDSCs separated by Transwell inserts to evaluate the functional importance of G-MDSC-derived IL-10 on A PMN killing of S. aureus by gentamicin protection assays (n = 10–12 biological replicates), B H2O2 production using OxiVision dye (n = 8 biological replicates), and C TNF production by intracellular staining (n = 4 biological replicates) (*p < 0.05; **, p < 0.01; ***p < 0.001; ****p < 0.0001; One-way ANOVA for each time point)