Fig. 1
From: Novel CH25H+ and OASL+ microglia subclusters play distinct roles in cerebral ischemic stroke
scRNA-seq revealed heterogeneity of microglial transcriptional states in the ischemic mice brain. A Microglia t-SNE representation. Each dot represents a single cell. B Expression of canonical marker genes of microglia (P2ry12, Hexb, Sparc). C Percentage of each MG cluster identified in B in different groups (Sham and Stroke). D Top highly expressed genes in MG 0–6 clusters. E Alluvial plot depicting the most affected GO for each cluster. Upregulated genes of each cluster were used for the enrichment analysis. Ribbon thickness indicates the number of genes per biological term. F Heatmap showing the GSVA score of ATM, SASP, DAM, and IRM for each cluster defined as the average normalize expression of the pathway-related genes. See Additional file 1: Table S5 for a list of DAM, IRM, IRM and ATM associated genes. (t-SNE t-distributed stochastic neighbor embedding, MG microglia, GO gene ontology, GSVA gene set variation analysis, ATM axon tract associated microglia, SASP senescence-associated secretory phenotype, DAM disease-associated microglia, IRM injury-responsive microglia)