Fig. 2
From: Novel CH25H+ and OASL+ microglia subclusters play distinct roles in cerebral ischemic stroke
Pseudo-time trajectory revealed homeostatic microglia subcluster differentiated into ISAM. A–C Monocle pseudo-time trajectory shows the progression of MG0-MG6. One branch was identified, leading to MG4 from MG0&2 (A). The color represents microglia clusters (A, C), pseudo-time (B). D Comparison of normalized gene expression in MG2 and MG0. E GSEA using DEGs of MG2 reveals positive enrichment for antigen processing and presentation of peptide antigen and cell adhesion mediated by integrin. F Gene expression heatmap across pseudotime. The DEGs are shown using heatmap with microglia in pseudo-time from root to termini. Terms associated with DEGs in the right columns. (ISAM ischemic sociated microglia, MG microglia, GSEA gene set enrichment analysis, DEG differentially expressed genes)