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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Novel CH25H+ and OASL+ microglia subclusters play distinct roles in cerebral ischemic stroke

Fig. 6

OASL+ microglia subset exhibits type I interferon upregulated signaling and was associated with increased infarct volume after stroke. A t-SNE representation of MG5 (left); violin plots showing the top up-regulated genes in MG5 (right). B Enriched GO terms in MG5. C Top predicted upstream transcriptional regulators of MG5 activation. D Schematic representation of the experimental design. E Cerebral blood flow was monitored using two-dimensional laser speckle imaging techniques on the left side before, during, and 3 days after distal middle cerebral artery occlusion (dMCAO). The values on the right side were expressed as a percentage change from the baseline measurements taken prior to dMCAO. F Quantification of infarct volume 3 days after dMCAO of young (3 months) and aged (21 months) mice. n = 6 per group. G Representative images of microglia in adult brain and aged brain after ischemic stroke. Scale bars, 30 μm. H OASL+ quantified in microglia (IBA1+) in the brain of young (3 months) and aged (21 months) mice of sham and ischemic stroke mice (young, n = 12; aged, n = 12; two-sided t test; mean ± SD). I Correlation between OASL+ microglia proportion and infarct volume of tMCAO and dMCAO. Data are presented as means ± SD. (t-SNE t-distributed stochastic neighbor embedding, MG microglia, GO gene ontology, DEG differentially expressed genes, IBA-1 ionized calcium binding adapter molecule 1, OASL 2'–5'-oligoadenylate synthetase-like, tMCAO transient middle cerebral artery occlusion, dMCAO distal middle cerebral artery occlusion; ANOVA analysis of variance)

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