Fig. 3

Treatment of SCA1 mice with JNK inhibitor abolishes c-Jun phosphorylation in reactive Bergmann glia. A Immunostaining of WT (vehicle/JNK inhibitor) and SCA1 (vehicle/JNK inhibitor) treated cerebellum with Bergmann glia (BG)-specific S100 (red) along with c-Jun-pS63 antibody (green). Scale bar = 100 μm. B Immunostaining of WT (vehicle/JNK inhibitor) and SCA1 (vehicle/JNK inhibitor) treated cerebellum with Purkinje cell-specific calbindin (Calb) antibody along with c-Jun-pS63 antibody. C-Jun-pS63-positive BG cells (green) sit adjacent to the Purkinje cells (red). Scale bar = 100 μm. C Quantification of the percentage of BG cells positive for c-Jun-pS63, as shown in A. ***P < 0.001, one-way ANOVA with Bonferroni’s multiple comparison test. Inhib inhibitor