Table 1 Overview of recent studies with single-cell analysis of meningeal immunity
From: Meningeal immunity and neurological diseases: new approaches, new insights
Single-cell technology | Tissue origin | Single-cell isolation | Protocol | References |
|---|---|---|---|---|
scRNA-seq | Leptomeninges from WT and EAE mice models | FACS sorting of CD45+ immune cells | CEL-Seq2 | 2019 [37] |
Dural and subdural meninges from WT C57BL/6 mice | FACS sorting of CD45+ immune cells | 10 × Chromium | 2019 [18] | |
Dural meninges from young and old WT C57BL/6 mice | FACS sorting of CD45+CD3e+TCRβ+ T cells | 10 × Chromium | 2021 [26] | |
Dural meninges from young and old WT C57BL/6 mice | FACS sorting of CD45−CD31−CD13+ mural and CD45−CD31+ endothelial cells | 10 × Chromium | ||
Whole dural meninges from young and old WT C57BL/6 mice | FACS sorting of DAPI− cells | 10 × Chromium | ||
Dural sinuses from WT C57BL/6 mice | FACS sorting of CD45+CD11b+Ly6G− myeloid cells | 10 × Chromium | ||
Whole dural meninges from WT C57BL/6 mice | FACS sorting of DAPI− cells | 10 × Chromium | 2021 [14] | |
Spinal cord tissue from EAE parabionts | FACS sorting of DAPI−CD45hiIV-CD45−GFP− and DAPI−CD45hiIV-CD45−GFP+ singlets | 10 × Chromium | ||
Spinal cord tissue from WT parabionts after spinal cord injury | FACS sorting of DAPI−CD45hiIV-CD45−GFP− and DAPI−CD45hiIV-CD45−GFP+ singlets | 10 × Chromium | ||
Dural meninges from WT C57BL/6 mice | Unsorted | 10 × Chromium | 2021 [15] | |
Dural meninges and subdural meninges from WT C57BL/6 mice | FACS sorting of CD45+CD45iv− tissue-resident leukocytes | 10 × Chromium | 2021 [24] | |
Six scRNA-seq data set integration | FACS sorting | 10 × Chromium; MARS-seq | 2022 [47] | |
scBCR-seq | Dural meninges from young and old C57BL/6 mice | Unsorted | 10 × Chromium | 2021 [15] |
Dural meninges from unimmunized and EAE mice models | Unsorted | 10 × Chromium | 2021 [24] | |
CyTOF | Mixed brain, enveloping meninges and choroid plexus from WT C57BL/6 mice | Unsorted | CyTOF1 mass cytometer | 2017 [19] |
Mixed brain and enveloping meninges from WT, geriatric, and EAE mice models | Unsorted | Third-generation Helios mass cytometer | 2018 [20] | |
Mixed CNS parenchyma and enveloping meninges from WT, EAE, and HD mice models | Unsorted | CyTOF2 mass cytometer | 2018 [21] | |
Mixed brain, enveloping meninges and choroid plexus from WT C57BL/6 mice | Unsorted | CyTOF1 mass cytometer | 2018 [22] | |
Dura mater from WT C57BL/6 mice | Unsorted | CyTOF2 mass cytometer | 2021 [15] |