Fig. 3

Administration of dBET6 alleviates LD-induced retina degeneration. A In vivo OCT shows retinal thickness and lamination with indicated treatment. LD led to altered reflectance in the ONL, which was reversed by dBET6 treatment. Lower panel: heat maps show the average retinal thickness. Scale bar: 100 μm. B Quantification of retinal thickness in the 3 mm and 6 mm circles. *: p < 0.05, ***: p < 0.0005, ****: p < 0.0001, one-way ANOVA, Tukey’s test; n = 6 mice for vehicle, n = 5 mice for LD + vehicle or LD + dBET6. C HE staining shows retinal structure with indicated treatment. Scale bar: 100 μm. n = 3 eyes for each group. D TUNEL staining shows retinal cell death with indicated treatment. Upper panel: TUNEL signals were indicated by white fluorescence. Lower panel: overlapping images of TUNEL and DAPI (nuclei). Scale bar: 20 μm. E Quantification of TUNEL-positive retinal cells in ONL of indicated treatment. For each group, four regions were randomly selected and the percentage of TUNEL-positive cells were calculated over DAPI-positive cells within ONL. ****: p < 0.0001, n = 3 eyes for each group, One-way ANOVA, Tukey’s test