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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage

Fig. 5

dBET6 inhibits LPS-induced microglia activation and migration. A WB analysis shows the indicated protein expression. BV2 cells were treated without (CTRL) or with dBET6 at the indicated concentration for 2 h and then total proteins were extracted for WB analysis. The lower panel shows quantification result of WB. ns not significant, *: p < 0.05, One-way ANOVA, Tukey’s test, n = 3 per group. B qRT-PCR analysis shows the indicated gene expression. BV2 Cells were left untreated (CTRL) or treated with the indicated drugs for 24 h before analysis. For LPS/ IFNγ, combined LPS (10 ng/ml) and IFN-γ (20 ng/ml) were used. For dBET6 treatment, 100 nM dBET6 was used. ns not significant, **: p < 0.01, ***: p < 0.0005, ***: p < 0.0001. One-way ANOVA, Tukey’s test, n = 3 per group. C High-content image analysis was used to access BV2 motility with the indicated treatment. Cell mobility was recorded in live BV2 cells for 8 h. Each cell migration route was labeled. Drug concentrations were the same as described in B. D Quantification results of cell migration. For each group, at least 300 cells were recorded. ****: p < 0.0001. One-way ANOVA, Tukey’s test

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