Fig. 6

LD led to activation of cGAS-STING innate immunity in mouse retina. A–D Mice were expose with or without bright light and retinas were collected 48 h after exposure. Total RNAs or proteins were extracted and subjected to RNA-sequencing or WB analysis or IHC analysis, respectively. n = 4 for each group. A Gene Ontology (GO) analysis shows significant up- or down-regulated biological processes. Numbers indicate log10 and − log10 of the p. adjust. B Gene set enrichment analysis (GSEA) profiles demonstrate significant enrichment of gene sets associated with indicated pathway in light-exposed mouse retinas compared to control retinas. C Heat map shows increased cGAS-STING genes in LD group as compared with CTRL. p < 0.05. D WB analysis shows indicated protein level in mouse retinas. Right panels show the quantification results of the presented WB and a repeated WB not shown. *: p < 0.05, **: p < 0.01, ***: p < 0.001, n = 4 per group, unpaired t-test. E The DNA was labeled by anti-dsDNA antibody. Note that the cytosolic DNA seems to be engulfed by IBA1-positive microglia/macrophages in the ONL. Scale bar: 50 and 10 μm