Table 1 Key reagents and resources used in this study

Antibodies

BRD2 polyclonal antibody

Proteintech

cat#22236-1-AP

Wes 1:40

BRD3 polyclonal antibody

Proteintech

cat#11859-1-AP

Wes 1:40

Rabbit polyclonal to Brd4

Abcam

cat# ab84776

Wes 1:40;WB 1:1000

Rabbit monoclonal [EPR16588] to IBA1

Abcam

cat#ab178846

WB 1:1000;IF 1:200

Beta tubulin mouse monoclonal antibody

Proteintech

cat#66240-1-Ig

Wes 1:10,000;WB 1:5000

Anti-rabbit IgG (H + L), F(ab′)2 fragment (Alexa Fluor® 488 conjugate)

Cell Signaling Technology

cat# 4412

IF 1:500

Anti-mouse IgG (H + L), F(ab′)2 fragment (Alexa Fluor® 594 conjugate)

Cell Signaling Technology

cat# 8890

IF 1:500

Anti-rabbit IgG (H + L), F(ab′)2 fragment (Alexa Fluor® 594 conjugate)

Cell Signaling Technology

cat# 8889

IF 1:500

Rabbit polyclonal to GFAP

Affinity Bio sciences

cat# DF6040

WB 1:1000

GFAP polyclonal antibody

Proteintech

Cat No. 16825-1-AP

IF 1:200

cGAS (D3O8O) rabbit mAb (mouse specific)

Cell Signaling Technology

cat#31659

WB 1:1000

TBK1/NAK (D1B4) rabbit mAb

Cell Signaling Technology

cat#3504

WB 1:1000

Phospho-TBK1/NAK (Ser172) (D52C2) XP® rabbit mAb

Cell Signaling Technology

cat#5483

WB 1:1000

p-Histone H2A.X (Ser 139) anti-mouse

Santa Cruz Biotechnology

cat#sc-517348

WB 1:1000

STING (D1V5L) rabbit mAb (rodent preferred)

Cell Signaling Technology

cat#50494

WB 1:1000;IF 1:200

CoraLite®594-conjugated TMEM173/STING polyclonal antibody

Proteintech

CL594-19851-100UL

IF 1:200 for retinal flat mount

TMEM173/STING monoclonal antibody

Proteintech

cat#66680-1-Ig

IF 1:200

Rabbit polyclonal to CD86

Affinity Bio sciences

cat# DF6332

WB 1:1000;IF 1:200

dsDNA Marker (HYB331-01)

Santa Cruz Biotechnology

cat#sc-58749

IF 1:200

CoraLite®594-conjugated TMEM173/STING polyclonal antibody

Proteintech

cat#CL594-19851

IF 1:200

CoraLite® Plus 488-conjugated IBA1 polyclonal antibody

Proteintech

cat#CL488-10904

IF 1:200

APC anti-mouse CD86 (GL1)

Proteintech

cat#APC-65068

IF 1:200

Chemicals, peptides, and recombinant proteins

PEG300

Selleck

cat#S6704

Tween-80

BioFroxx

cat#1716

DMSO

MP Biomedicals

cat#196055

LPS

Sigma

cat#L4391

TUNEL BrightRed apoptosis detection kit

Vazyme

cat#A113

dBET6

Selleck

cat#S8762

MG132

Selleck

cat#S2619

Experimental models: cell lines

661W

Huangxuan Shen Lab

N/A

BV2

ATCC

Experimental models: organisms/strains

Mouse: C57BL/6: C57BL/6J

Sun Yat-Sen University

C57BL/6J

Laboratory Animal Center

Mouse: BALB/c

Sun Yat-Sen University

BALB/cJ

Laboratory Animal Center

Published sequencing data

Single-cell RNA-seq Data

GitHub

https://github.com/jiewwwang/Single-cell-retinal-regeneration

Bulk-RNA Data

NCBI

PRJNA916821;PRJNA954826

Software and algorithms

GraphPad Prism 8.0.2

GraphPad software

https://www.graphpad.com/

ImageJ 1.46/Fiji

NIH

https://imagej.nih.gov/ij/

SRA Tools

NCBI

https://github.com/ncbi/sra-tools

SAMTools

Li H et al. (2009)

http://www.htslib.org/

featureCounts

Yang Liao et al. (2013)

http://www.bioconductor.org

DESeq2

Michael I Love et al. (2014)

http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html

ComplexHeatmap

Zuguang Gu et al. (2016)

http://www.bioconductor.org/packages/devel/bioc/html/ComplexHeatmap.html

clusterProfiler

Guangchuang Yu et al. (2012)

http://bioconductor.org/packages/release/bioc/html/clusterProfiler.html

Seurat

Hao*, Hao*, et al. Cell (2021)

Tools for Single Cell Genomics · Seurat (satijalab.org)