Fig. 1

Schematic outlining the study design. The study consisted of two groups: control (vehicle, n = 9) and LPS (n = 8). The solid lines show the timing of the lipopolysaccharide (LPS)/vehicle infusions which were given over 2 min at increasing doses (300 ng, 600 ng, and 1200 ng). Controls received an equivalent volume of vehicle (saline) during the infusion period. Continuous electroencephalogram (EEG) recordings were performed throughout the experiential period. At 96 h, brains were collected for Golgi staining to examine neuronal arborisation and numbers of dendritic spines, immunohistochemistry to assess neuroinflammation, neuronal numbers and cortical area and mRNA assessment of proinflammatory proteins. Boxes indicate regions of interest used for immunohistochemical and Golgi analysis. Image source:[86]