Table 1 Primer sequences for qPCR
Gene | Species | Accession number | Primer sequence | Amplicon length, nt |
|---|---|---|---|---|
18S | Rat | NR_046237.1 | 5ʹ-GTAACCCGTTGAACCCCATT-3ʹ 3ʹ-CCATCCAATCGGTAGTAGCG-5ʹ | 151 |
IL1A | Sheep | NM_001009808.1 | 5ʹ-GTCCATACATGACGGCTGCTA-3ʹ 3ʹ-GGTGTCTCAGGCATCTCCTTAT-5ʹ | 184 |
IL1B | Sheep | NM_001009465.2 | 5ʹ-CGATGAGCTTCTGTGTGATG-3ʹ 3ʹ-CTGTGAGAGGAGGTGGAGAG-5ʹ | 121 |
IL6 | Sheep | NM_001009392 | 5ʹ-CGCAAAGGTTATCATCATCC-3ʹ 3ʹ CCCAGGAACTACCACAATCA-5ʹ | 108 |