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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Circular RNA Tmcc1 improves astrocytic glutamate metabolism and spatial memory via NF-κB and CREB signaling in a bile duct ligation mouse model: transcriptional and cellular analyses

Fig. 2

circTmcc1 regulates astrocytic function in BDL mice cortex. A Genomic information of circTmcc1 in mouse Tmcc1 locus (mm10). This information and PhastCons data were obtained from the UCSC Genome Browser. The siRNAs were designed to bind to the back-splicing junction of circTmcc1. Confirmation of the circular formation of circTmcc1 with RNase R treatment. B Comparison of mRNA expression of glutamate and glutamine synthesis-related genes (Gls, Glul) in circTmcc1-downregulated C8-D1a cells. The data are reported as mean ± SEM (n = 3). C Measurement of intracellular glutamate and glutamine levels in C8-D1a cells after circTmcc1 knockdown. The data are expressed as three independent experiments in duplicate. D Measurement of GABA concentration in C8-D1a cells after circTmcc1 knockdown. The data are expressed as three independent experiments in duplicate. E The measurement of the expression of Gl syn and AQP-4 protein in circTmcc1-downregulated C8-D1a cells. The expression changes are presented as the mean ± SEM (n = 3). An unpaired two-tailed t-test with Welch’s correction was used for statistical analysis. ns, not significant, *p < 0.05, **p < 0.01

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Journal of Neuroinflammation

ISSN: 1742-2094