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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Circular RNA Tmcc1 improves astrocytic glutamate metabolism and spatial memory via NF-κB and CREB signaling in a bile duct ligation mouse model: transcriptional and cellular analyses

Fig. 5

circTmcc1 controls the secretion of proinflammatory mediators in astrocytes and modulates neuronal complexity. A Immunoblot measurement of the cytokine and chemokine levels of control siRNA and circTmcc1-siRNA-transfected C8-D1a cells. Differential level histogram of cytokines and chemokines between control siRNA and circTmcc1-siRNA-treated cells. B The expression level of proinflammatory cytokine- and chemokine-related genes (Ccl2, Tnf-α, IL-6, IL-1β, and Cox2) in C8-D1a cells following circTmcc1 knockdown. These expression changes are described as the mean ± SEM (n = 3). C The changes in the protein of NF-κB following circTmcc1 knockdown in C8-D1a cells are described as the mean ± SEM (n = 3). D The gene expression changes (Rbfox3, Map2, and Syp) related to neuronal and synaptic function were measured and are presented as the mean ± SEM (n = 3). E Changes in the neurite length of the C8-D1a-conditioned media (CM)-treated differentiated Neuro-2A cells. F Immunocytochemical images of SYP and PSD-95 expression in circTmcc1-downregulated C8-D1a cells. The representative cells from three independent cultures (n = 3). An unpaired two-tailed t-test with Welch’s correction was used for statistical analysis. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.005

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