Molecular patterns from a human gut-derived Lactobacillus strain suppress pathogenic infiltration of leukocytes into the central nervous system

Multiple sclerosis (MS) is an inflammatory demyelinating disease that affects 2.5 million people worldwide. Growing evidence suggests that perturbation of the gut microbiota, the dense collection of microorganisms that colonize the gastrointestinal tract, plays a functional role in MS. Indeed, specific gut-resident bacteria are altered in patients with MS compared to healthy individuals, and colonization of gnotobiotic mice with MS-associated microbiota exacerbates preclinical models of MS. However, defining the molecular mechanisms by which gut commensals can remotely affect the neuroinflammatory process remains a critical gap in the field. We utilized monophasic experimental autoimmune encephalomyelitis (EAE) in C57BL/6J mice and relapse-remitting EAE in SJL/J mice to test the effects of the products from a human gut-derived commensal strain of Lactobacillus paracasei (Lb). We report that Lb can ameliorate preclinical murine models of MS with both prophylactic and therapeutic administrations. Lb ameliorates disease through a Toll-like receptor 2-dependent mechanism via its microbe-associated molecular patterns that can be detected in the systemic circulation, are sufficient to downregulate chemokine production, and can reduce immune cell infiltration into the central nervous system (CNS). In addition, alterations in the gut microbiota mediated by Lb-associated molecular patterns are sufficient to provide partial protection against neuroinflammatory diseases. Local Lb modulation of the gut microbiota and the shedding of Lb-associated molecular patterns into the circulation may be important physiological signals to prevent aberrant peripheral immune cell infiltration into the CNS and have relevance to the development of new therapeutic strategies for MS.


Background
Multiple sclerosis (MS) is an immune-mediated disorder of the central nervous system (CNS) that is characterized by demyelination, inflammation, and axonal damage [1]. While a definitive cause for MS remains elusive, recent attention has turned to the gut microbiota as an environmental factor that may significantly impact diseases. Studies of the fecal microbiome of MS patients have revealed specific bacterial taxa that are reproducibly altered in MS patients compared to healthy controls [2,3]. Indeed, the transfer of MS-associated microbiota into gnotobiotic mice exacerbates experimental autoimmune encephalomyelitis (EAE), the most common preclinical model of MS, suggesting that the microbiota can play a functional role in diseases [3,4]. Mechanistically, a growing catalog of microbes and microbial products have been identified that have the ability to skew the neuroinflammatory response away from a proinflammatory phenotype and toward an anti-inflammatory phenotype [5,6].
Here, we report that a human gut-derived commensal strain of Lactobacillus (Lb) is able to suppress the infiltration of leukocytes into the CNS during EAE, independent of skewing the CNS effector T cell response. The protective effect of Lb is dependent on host recognition of Lb-derived microbe-associated molecular patterns (MAMPs) via Toll-like receptor (TLR) 2, as TLR2deficient mice are not protected by Lb treatment. Furthermore, intestinal microbiota transplants from mice treated with Lb-associated molecular patterns partially ameliorate EAE in recipient mice, suggesting that downstream changes in the gut microbiota also contribute to the effects of Lb. Our findings provide evidence of how gut-derived MAMPs can regulate autoimmunity in the CNS and may support new therapeutic strategies in MS.

Mice
Wild-type C57BL/6J, TLR2-deficient C57BL/6, and wildtype SJL/J mice were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). TLR9-deficient C57BL/6 mice were a kind gift from Dr. Shizuo Akira via Dr. Stella Elkabes. Mice of both sexes were studied. All animal experiments were reviewed and approved by the University of Utah Institutional Animal Care and Use Committee and conducted in accordance with the guidelines prepared by the Committee on Care and Use of Laboratory Animals, Institute of Laboratory Animals Resources, National Research Council. Animals were bred and maintained in the pathogen-free Comparative Medicine Center at the University of Utah on a 12-h light/12h dark cycle at 22°C. Germ-free mice were maintained in sterile isolators and verified for germ-free status by plating and PCR of feces. Food and water were available ad libitum. All efforts were made to minimize suffering.

EAE induction
C57BL/6 mice were injected subcutaneously in the flanks with 200 μL of 1 mM myelin oligodendrocyte glycoprotein (MOG) 35   peptide and SJL/ J mice injected with PLP 139-151 peptide were weighed and scored regularly for clinical signs of disease. Clinical scoring was as follows: 0, no clinical disease; 1, loss of tail tonicity; 2, mild hind leg paralysis with no obvious gait disturbance; 3, mild leg paralysis with gait disturbance; 3.5, unilateral hind limb paralysis; 4, bilateral hind limb paralysis; and 5, moribund or dead. Plots of the clinical score over time include EAE-induced mice that do not develop disease, when applicable. If the mice were paralyzed to the point where they could not feed or groom themselves (moribund), or they lost 20% of their body weight, the mice were euthanized via inhaled anesthetic.

Flow cytometry
Mice were euthanized through inhaled anesthetic and perfused with phosphate-buffered saline (PBS). Brain and spinal cord tissues were harvested and enzymatically dissociated with collagenase (MilliporeSigma, St. Louis, MO, USA) and DNase I (Roche, Basel, Switzerland) and subsequently mechanically dissociated by vigorous pipetting. Leukocytes were enriched by Percoll (Millipore-Sigma) density gradient centrifugation. Cells were treated with Fc blocker (BioLegend, San Diego, CA, USA), stained with the indicated anti-mouse antibodies for 30 min at 4°C [V500 anti-mouse CD45 (BD Bioscience, San Jose, CA, USA), APC anti-mouse CD11b (eBioscience, San Diego, CA, USA)], and analyzed by flow cytometry. CNSderived cells were stained and analyzed individually for each mouse. Live cells were determined by forward and side scatter fluorescence on a BD LSRFortessa X-20 Cell Analyzer (BD Bioscience). Flow cytometry data analysis was performed using the FlowJo software (FlowJo, Ashland, OR, USA).

Histological analysis
Mice were euthanized through inhaled anesthetic and perfused with PBS followed by 4% paraformaldehyde phosphate-buffered solution. For EAE mice, the spinal cords were harvested, divided into 12 transverse portions, embedded in paraffin, and cut into 4-μm-thick tissue sections. To visualize myelin, the sections were stained with Luxol fast blue. Slides were scanned using the Pannoramic MIDI digital slide scanner, visualized using CaseViewer 2.2 digital microscope software, and quantified using the QuantCenter image analysis platform (3D HISTECH Ltd., Budapest, Hungary). The extent of demyelination was calculated in annotated white matter tracts of the spinal cord by subtracting the percent dark blue (myelinated) area of the total white matter area from 100%.

Adoptive transfer
Congenically labeled CD45.1 + leukocytes from the spleen and mesenteric lymph nodes were isolated 6 days post-EAE induction of donors by mechanical dissociation and enriched using ammonium chloride to lyse red blood cells. Leukocytes were suspended in PBS and transferred to CD45.2 + -recipient mice (10 8 leukocytes per mouse) by retroorbital injection, with adoptive transfer-naive mice injected retroorbitally with PBS, 1 day prior to EAE induction of recipients.

Culture of bacteria
Lactobacillus sp. was grown aerobically in De Man, Rogosa, and Sharpe (MRS) broth (VWR, Denver, CO, USA) at 37°C, and identity was confirmed using 16S rRNA-specific PCR followed by sequencing. Heat-killed (HK) Lb was obtained by heating bacteria to 80°C for 30 min, and killing was confirmed by plating. Colonyforming units (CFUs) of Lb prior to heat-killing was obtained to ensure the equivalent of 10 9 CFUs were being delivered. In experiments in which live Lb was compared to HK Lb, a bulk preparation of Lb was made, of which a portion underwent the heat-killing procedure. Bulk preparations of Lb were grown overnight, and glycerol stocks were used through the duration of an experiment. Lb-conditioned media were obtained by growing an overnight culture of Lb, collecting the supernatant, and filter sterilizing the solution through a 0.45-μm filter. The absence of viable cells was confirmed by plating.
Blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) permeability assay BBB and BSCB permeabilities were measured by injecting 100 μL of 1% Evans blue dye (in 0.9% saline, 0.22 μm filtered) retroorbitally into mice. The dye was allowed to circulate for 2-4 h before sacrificing the mice. The mice were perfused with PBS for 3 min to clear the dyecarrying blood from the vessels. Brain and spinal cord tissues were weighed and immersed in formamide overnight at 60°C to elute the dye. The amount of dye per gram tissue was quantified by spectrophotometry.

Colonization with PBS-and HK Lb-associated microbiota
Conventionally colonized C57BL/6 mice (n = 1 donor mouse per group) were treated daily with either PBS or HK Lb for 4 weeks. Donor mice were sacrificed, and the intestinal contents (small intestine to colon) were harvested and resuspended in PBS under anaerobic conditions. Germ-free mice (9-10 weeks old) were colonized with either PBS-or HK Lb-associated microbiota (n = at least 10 recipient mice per group) via oral gavage and were induced to develop EAE 6 weeks after colonization.

TLR2 ligand detection
HEK-Blue mTLR2 cells (InvivoGen, San Diego, CA, USA) were used according to the manufacturer's instruction to determine the serum concentrations of TLR2 ligands. After passaging according to the manufacturer's instruction, a flat-bottom 96-well plate was loaded with 25,000 cells per well. Then, 20 μL of PBS, mouse serum, or serial dilutions, starting from 0.1 μg/mL, of Pam3CSK4, a synthetic triacylated lipopeptide, were added to each well. Cells were incubated for 6 h, spun down, and supernatant isolated. Twenty microliters of supernatant was then incubated with 180 μL of QUANTI-Blue solution (InvivoGen) for 15 min. Optical density at 620 nm was then measured using a Biotek Synergy H1M plate reader.

Statistical analysis
Statistical analysis was performed using GraphPad Prism 7 (GraphPad Software, San Diego, CA, USA). Statistical significance was determined using one-way ANOVA with Tukey's multiple comparisons test to compare the multiple groups or Student t test to compare the two groups. Log-rank test was used to compare the EAE incidence between the groups. P < 0.05 was used to define statistical significance.

Live Lb is not necessary to suppress EAE
Previously, through a large-scale screen of EAE-induced mice, we identified Lactobacillus sp. as being inversely correlated with disease severity and went on to demonstrate that supplementation of a specific strain of L. paracasei, Lb, was sufficient to ameliorate EAE [7]. Given that Lactobacillus has been shown to ameliorate EAE in a species-and strain-dependent manner [8], we first asked whether the protective capacity of Lb was conserved among other L. paracasei strains. To do this, we treated C57BL/6J mice daily via oral gavage with 10 9 CFUs with Lb and four other strains of L. paracasei isolated from distinct origins (Fig. 1a). We found that the ability to protect against monophasic EAE is largely conserved among different L. paracasei strains (Fig. 1b, c). Among the strains that resulted in a significant decrease in disease incidence and severity, Lb was unique in its human gut origin and was used in subsequent experiments. In order to investigate the Lb-derived products required to ameliorate EAE, we treated mice daily with either 10 9 CFUs of live Lb, an equivalent preparation of HK Lb, or PBS via oral gavage starting 2 weeks prior to EAE induction (Fig. 1a). Monitoring disease incidence and severity, we found that, as before, live Lb-treated animals exhibited lower EAE incidence and severity compared to PBS-treated animals (Fig. 2a, b). Surprisingly, HK Lb-treated animals also exhibited lower EAE incidence and severity compared to PBS-treated animals with protection similar to live Lb-treated animals (Fig. 2a, b). Analysis of spinal cord tissue revealed that both live Lb-and HK Lbtreated mice had less demyelination compared to PBStreated mice with no difference in demyelination between live Lb-and HK Lb-treated mice (Fig. 2c, d). To further probe whether soluble products secreted by viable Lb contributed to the protective mechanism of Lb, we assessed whether cell-free Lb-conditioned media could limit EAE. We found that mice treated with Lb-conditioned media had the same incidence of EAE and had more severe disease compared to mice treated with sterile De Man, Rogosa, and Sharpe (MRS) media (Fig. 2e, f).
We also tested whether HK Lb could be used after the onset of EAE to ameliorate disease (Fig. 1a). We utilized a RR-EAE model in SJL/J mice and initiated treatment with either HK Lb or PBS after the initial attack. We found that therapeutic treatment with HK Lb decreased the severity of the subsequent relapse in RR-EAE (Fig. 3), Fig. 2 Heat-killed (HK) Lb is sufficient to ameliorate EAE. a EAE clinical scores and b incidence for C57BL/6J mice orally administered vehicle (PBS), HK Lb, or live Lb daily starting 14 days prior to induction (n = at least 10 mice per group). c Quantification of demyelination by Luxol fast blue (LFB) staining in the spinal cord cross-sections at 30 DPI (n = at least 8 mice per group and at least 6 sections per mouse). d Representative LFB staining of the thoracic spinal cord sections from treated mice. e EAE clinical scores and f incidence for C57BL/6J mice orally administered MRS media or Lb-conditioned media starting 14 days prior to induction (n = at least 11 mice per group). Data are presented as mean ± standard error of the mean (SEM) and are representative of two independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. ns, not significant; AUC one-way ANOVA with Tukey's multiple comparisons test for a, log-rank test for b and f, one-way ANOVA with Tukey's multiple comparisons test for c, and AUC Student t test for e although the effect of HK Lb was not as pronounced as that observed with prophylactic treatment in monophasic EAE (Fig. 2a). Together, these data demonstrate that the protective effect of Lb is largely conserved among diverse L. paracasei strains and that endogenously expressed molecular patterns of Lb are sufficient to prophylactically and therapeutically ameliorate EAE.

HK Lb limits CNS-infiltrating leukocytes by downregulating circulating chemokines
To determine the effect of Lb treatment on monophasic EAE, we isolated leukocytes from the brain and spinal cord of C57BL/6J mice treated with live Lb, HK Lb, and PBS and characterized the CNS infiltrate via flow cytometry. Live Lb-and HK Lb-treated mice had fewer infiltrating macrophages and lymphocytes in both the brain and spinal cord compared to PBS-treated mice (Fig. 4a, b). We also analyzed the proportions of CD4 + IFNγ + T cells, CD4 + IL-17A + T cells, and T regulatory cells (Tregs) in the CNS and found no difference between Lb-treated and PBS-treated mice (Fig. 5a-c). Importantly, the number of leukocytes in the spleen between HK Lb-treated mice and PBS-treated mice was not different (Fig. 4c). These results provide evidence that Lb impedes leukocyte migration into the CNS independent of effector T cell modulation.
Given the reduction in CNS-infiltrating leukocytes in Lb-treated mice, we obtained the plasma from these mice and measured the level of various chemokines by a multiplex bead-based assay. We observed a reduction in select chemokines CCL3, CCL4, CXCL5, and CXCL13 in HK Lb-treated mice (Fig. 4d). We also tested the permeability of the BBB and BSCB of these mice by Evans blue dye. Mice treated with HK Lb did not differ in BBB and BSCB permeability compared to PBS-treated mice (Fig. 6a, b). Together, these data suggest that Lb decreases the production of chemokines to affect the infiltration of leukocytes into the CNS without affecting the physical permeability of the blood-CNS barrier.
While we did not observe a difference in the proportion of critical helper T cell subsets in the CNS with Lb treatment (Fig. 5a-c), we directly tested whether Lb alters the function of leukocytes to protect against EAE by adoptively transferring leukocytes from the spleen and mesenteric lymph nodes of HK Lb-treated, EAE-induced donor mice into Lb-naive recipient mice 1 day prior to EAE induction. EAE-induced recipient mice that received leukocytes from Lb-treated donors exhibited similar EAE incidence and severity as mice that received leukocytes from PBS-treated donors as well as adoptive transfer-naive mice (Fig. 7a, b). Flow cytometric analysis of CNS leukocytes revealed that donor-derived CD45.1 + leukocytes from HK Lb-treated donors were trending to be less prevalent in the brain compared to leukocytes from PBS-treated donors (Fig. 7c). However, there was no difference in the prevalence of donor-derived leukocytes in the spinal cord (Fig. 7c). Importantly, the number of endogenous CD45.2 + leukocytes in the CNS (Fig. 7d) and spleen (Fig. 7e) was not different between the groups, and the number of donorderived CD45.1 + leukocytes in the spleen was also not different between the adoptive transfer recipients (Fig. 7f). Thus, Lb treatment likely ameliorates EAE via an immune cell-extrinsic mechanism. Host TLR2 signaling is necessary for the protective mechanism of Lb Since HK Lb is sufficient to suppress EAE, we sought to define the host pattern recognition receptors critical for sensing Lb-associated molecular patterns and leading to a protective phenotype. We hypothesized that TLR2 was a critical signaling node in the protective mechanism of Lb as TLR2 senses peptidoglycan, lipoteichoic acid, and lipoproteins that are major components of the Lb cell wall [9]. Indeed, when we assayed plasma for the presence of TLR2 ligands using TLR2 reporter cells, we found that HK Lb-treated animals had significantly higher concentrations of circulating TLR2 ligand compared to PBS-treated animals at 0 days post-induction (DPI) and a trending increase at 14 DPI (Fig. 8a). To test whether TLR2 was necessary for Lb-mediated regulation of neuroinflammation, we utilized TLR2-deficient mice and compared their response to HK Lb treatment to that of wild-type mice (Fig. 1a). TLR2-deficient mice treated with PBS exhibited similar disease incidence and severity to wild-type mice treated with PBS (Fig. 8b, c). However, TLR2-deficient mice treated with HK Lb did not exhibit significantly less severe disease as observed with wildtype mice receiving HK Lb (Fig. 8b, c). This was also corroborated by histological analysis of demyelination, revealing that while HK Lb-treated wild-type mice exhibited less demyelination than PBS-treated wild-type mice, no such protection was observed with HK Lbtreated TLR2-deficient mice (Fig. 8d, e). To test whether this effect was specific to TLR2, we also measured the response of TLR9-deficient mice to Lb treatment and found that TLR9-deficient mice retained the protective response to Lb treatment (Fig. 8f, g). Thus, TLR2 is specifically necessary for the protective mechanism of Lb.

HK Lb modulates the gut microbiota to reduce EAE incidence
Finally, we utilized a gnotobiotic approach to probe whether the protective effect of HK Lb was due to direct interaction with the host or an indirect mechanism via HK Lb interaction with other members of the gut microbiota (Fig. 1a). Because germ-free mice exhibit significantly less severe EAE compared to conventionally colonized mice and would be limited in discerning a protective effect of HK Lb directly, we instead compared the development of EAE in germ-free mice colonized with HK Lb-associated gut microbiota or PBS-associated gut microbiota [10]. As before, we treated conventionally colonized C57BL/6 mice daily with either PBS or HK Lb via oral gavage for 4 weeks. We then sacrificed these donor mice and colonized germ-free C57BL/6 mice with their intestinal contents. Monophasic EAE was induced in these mice 6 weeks after the initial colonization. While the overall disease severity was not different between mice colonized with the PBS-associated microbiota or the mice colonized with the HK Lb-associated microbiota (Fig. 9a), mice colonized with the HK Lbassociated microbiota had a significantly lower incidence of EAE (Fig. 9b). Thus, changes in the gut microbiota induced by HK Lb recapitulate some, but not all the features, of HK Lb treatment itself. HK Lb, therefore, likely interacts with both the host and the endogenous gut microbiota to limit CNS autoimmunity.

Discussion
Given its role in shaping the host immune response, among other physiological processes, significant effort has been devoted to investigating the role of the gut microbiota in MS. Lactobacillus sp. has been found to be significantly depleted in MS patients compared to controls [2,3], mirroring what we and others have observed in EAE [7,11]. Recently, it has been shown that myelin-specific T helper (Th)17 cells proliferate in the gut prior to the development of neurological symptoms in EAE and are associated with a decrease in gut-derived Lactobacillus [11]. Therefore, depletion of Lactobacillus may be an early event in MS pathogenesis. Mechanistically, Lactobacillus has been proposed to ameliorate EAE by inhibiting pathogenic Th1 and Th17 responses via tryptophan-derived indole metabolites [12][13][14] and supporting protective Treg responses through an interleukin-10-dependent process [8]. Our work provides an alternative mechanism in which Lactobacillus-associated molecular patterns are sufficient to protect against EAE in the absence of Lactobacillusderived metabolites by controlling leukocyte migration to the CNS and modulating the gut-resident microbiota. These are not mutually exclusive pathways as the diverse Lactobacillus species that have been shown to ameliorate EAE, including L. murinis, L. reuteri, L. In this study, we demonstrate that molecular patterns from Lb, a human gut-derived commensal strain of L. paracasei, are sufficient to suppress two different preclinical models of MS (Figs. 2 and 3). Comparing Lb to other L. paracasei strains, we show that protection against EAE is conserved among L. paracasei strains from diverse sources (Fig. 1). While we do not compare Lb to non-Lactobacillus strains, other groups have demonstrated that the administration of bacteria, including Escherichia coli MG1655 and segmented filamentous bacteria, can have a neutral or exacerbating effect on EAE [10,15]. Additionally, Lb-conditioned media exacerbate disease compared to sterile MRS media (Fig. 2e, f). These data argue that the protection we observe in EAE is specifically due to Lbassociated molecular patterns.
Locally, oral administration of HK Lb shifts the gutresident microbiota to reduce the incidence of EAE (Fig. 9b). It is not clear how Lb-associated molecular patterns alter the gut microbiota, but may involve altering the gut mucosal immune response or the HK Lb itself acting as a substrate for the growth of certain strains. Our gnotobiotic approach demonstrates that products from the microbes associated with HK Lb treatment are sufficient to reduce EAE incidence; however, mice colonized with HK Lb-associated microbiota that develop EAE exhibit more severe disease than mice colonized with PBS-associated microbiota. Combined, this results in no significant effect of HK Lbassociated microbiota on the overall severity of EAE and suggests that repeated HK Lb treatment is necessary for maximal protection (Fig. 9a). The discordant effects of colonization with HK Lb-associated microbiota suggest that HK Lb administration shifts the gut microbiota to prevent disease, but upon disease induction and in the absence of continued HK Lb administration, mice that breakthrough this tolerogenic state adopt more severe disease. Further work is needed to characterize the alterations in the gut microbiota induced by HK Lb and the mechanism by which HK Lb changes the gut microbiota. Still, these data imply that HK Lb both functionally alters the gut microbiota as well as directly interacts with the host as part of its protective mechanism.
Beyond the gut, Lb-associated molecular patterns are also found systemically, attenuating leukocyte infiltration into the CNS (Fig. 4a, b). Interestingly, disease protection conferred by Lb is not associated with modulation of Tregs, CD4 + IFNγ + T cells, or CD4 + IL-17A + T cells, which are key to EAE pathogenesis [16], nor is adoptive transfer of Lb-treated leukocytes sufficient to protect against disease (Figs. 5 and 7). This suggests that Lb functions through a leukocyte-extrinsic mechanism and that sustained exposure to Lb-associated molecular patterns is important to ameliorate EAE. Critically, we show that while the physical permeability of the BBB and BSCB is not affected by HK Lb treatment, HK Lbtreated animals exhibit decreased circulating chemokines (Figs. 4d and 6). These chemokines can be produced from non-leukocyte populations including astrocytes and endothelial cells and can facilitate extravasation of peripheral leukocytes into the CNS independent of the physical integrity of CNS barriers [17]. The importance of Lb-associated molecular patterns is further substantiated by the higher concentration of TLR2 ligands we observe in the circulation of HK Lb-treated animals and the requirement of host expression of TLR2 for Lbmediated protection (Fig. 8). During the course of EAE, gut permeability increases [15], and thus, even EAEinduced, PBS-treated animals likely have a variety of gut-derived MAMPs in circulation. Our work provides proof-of-principal that translocation of Lb-derived MAMPs, in particular, from the gut into systemic circulation can ameliorate CNS autoimmunity.
Why would circulating MAMPs regulate peripheral autoimmunity? One possible explanation is that regular exposure to microbiota-derived MAMPs functions in a rheostat-manner to tolerize the host immune response against inappropriate activation. Locally, gut commensal microbes can favor colonization using molecular patterns to modulate the host mucosal immune response [18], and systemically, MAMPs from the microbiota are constantly shed into the circulation under physiological conditions [19]. While the host has mechanisms to clear circulating MAMPs [20], MAMPs are constitutively present in the circulation and their function is not fully understood. A homeostatic level of circulating MAMPs appears to be critical for the development of the immune system [21], though excess circulating MAMPs lead to systemic inflammation [22]. With relevance to MS, systemic administration of a number of MAMPs, including polysaccharide A derived from Bacteroides fragilis and extracellular adherence protein of Staphylococcus aureus [23], has been shown to benefit preclinical models of neuroinflammation. Adding to these examples, we find that Lb-associated molecular patterns can impede CNS-infiltrating immune cells and ameliorate EAE. We propose that gut commensal Lactobacillus may be a major physiological source of circulating MAMPs as many strains express factors that confer bile and acid resistance, allow for direct adherence to the gut Fig. 7 Adoptive transfer of spleen and mesenteric lymph node leukocytes from HK Lb-treated mice does not protect against induced EAE. a EAE clinical scores and b incidence for adoptive transfer-naive C57BL/6 mice or mice receiving 10 8 leukocytes from either PBS-treated, EAE-induced donors or HK Lb-treated, EAE-induced donors at − 1 DPI (n = at least 4 mice per group). c Transferred CD45.1 + leukocytes and d endogenous CD45.2 + leukocytes in the CNS of recipient mice (n = 4 per group). e Endogenous CD45.2 + leukocytes and f transferred CD45.1 + leukocytes in the spleen of recipient mice (n = 4 per group). Data are presented as mean ± SEM. **P ≤ 0.01, ***P ≤ 0.001. ns, not significant; AUC one-way ANOVA Tukey's multiple comparisons test for a, log-rank test for b, and one-way ANOVA Tukey's multiple comparisons test for c to f b EAE clinical scores and c incidence for wild-type and TLR2-deficient C57BL/6 mice treated with PBS or HK Lb (n = at least 21 mice per group). d Quantification of demyelination by LFB staining in the spinal cord cross-sections at 30 DPI (n = at least 5 mice per group and at least 6 sections per mouse). e Representative LFB staining of the thoracic spinal cord sections from treated mice. f EAE clinical scores and g incidence for TLR9-deficient C57BL/6 mice treated with PBS or HK Lb (n = at least 14 mice per group). Data are pooled from two independent experiments and presented as mean ± SEM. *P ≤ 0.05, **P ≤ 0.01. ns, not significant; Student t test for a, AUC one-way ANOVA with Tukey's multiple comparisons test for b, log-rank test for c and g, one-way ANOVA with Tukey's multiple comparisons test for d, and AUC Student t test for f Fig. 9 HK Lb reduces EAE incidence by altering the gut microbiota. a EAE clinical scores and b incidence for germ-free C57BL/6 mice colonized with PBS-associated or HK Lb-associated microbiota (n = at least 10 mice per group). Data are presented as mean ± SEM. *P ≤ 0.05. ns, not significant; AUC Student t test for a and log-rank test for b epithelium, and thus would be better positioned to have their MAMPs translocate into circulation [24].
In support of a major role for Lb-associated molecular patterns in conferring resistance against neuroinflammation, we identify TLR2 as a critical host pattern recognition receptor in the protective mechanism of Lb. MS patients exhibit increased expression of TLR2 in peripheral blood mononuclear cells, cerebrospinal fluid cells, and demyelinating lesions [25], and in line with our own preclinical results, MS patients also exhibit decreased microbiota-derived TLR2 ligands in the blood [26]. Additionally, post-mortem analyses of whole brains from MS patients have identified the presence of peptidoglycan in demyelinating lesions [27,28], evidencing the ability of MAMPs to reach the CNS parenchyma in a neuroinflammatory context. However, investigations into the functional role of TLR2 in MS have produced mixed results. TLR2-deficient mice have been shown to develop either less severe [29] or equivalent [30] EAE to wildtype mice, though this variability may be due to the differences in the microbiota between mouse strains. In fact, TLR2-deficient mice have been reported to display a distinct gut microbiota compared to wild-type mice of the same background, likely due to the differences in the immune response to commensal organisms, with TLR2deficient mice containing higher levels of Lactobacillus than wild-type mice [31]. We strived to minimize variance in the gut microbiota between mouse strains by cohousing mice of different genotypes starting at weaning (4 weeks of age) and continuing through the duration of our experiments to demonstrate the necessity of TLR2.
In apparent opposition to our results, adjuvants used to induce EAE contain TLR ligands, including molecules recognized by TLR2. Additionally, Staphylococcus aureus-derived peptidoglycan, which can act as a TLR2 ligand [32], has previously been shown to be a sufficient adjuvant to induce EAE [33]. However, the acute high dose subcutaneous injection of adjuvant is a substantially different context than the chronic low-dose oral administration of HK Lb in this work. While it is possible that these adjuvants may have effects on TLR signaling in our in vivo experiments, this is controlled for in our experimental paradigm as both PBS-treated and Lb-treated mice receive adjuvant. Our data is in agreement with other studies that have demonstrated decreased EAE severity with repeated administration of TLR2 agonists, synthetic or microbiota-derived [34]. These results suggest that while acute TLR2 activation can exacerbate neuroinflammation, prolonged repeated TLR2 signaling may dampen the response to subsequent inflammatory stimuli [34]. Mechanistically, our work demonstrates that daily HK Lb treatment decreases circulating proinflammatory chemokines CCL3, CCL4, CXCL5, and CXCL13 in response to EAE induction (Fig. 4d).
Interestingly, these chemokines and/or their respective receptors have been observed to be elevated in MS patients [35], suggesting that Lb-associated molecular patterns result in TLR2-dependent tolerance of a clinically relevant pathway.

Conclusions
In summary, our findings demonstrate that gut-derived Lb-associated molecular patterns can limit leukocyte migration into the CNS and ameliorate neuroinflammation. While further work is necessary to determine the specific Lb-associated molecular patterns responsible for protection in EAE, beneficial neuroimmunomodulation appears to be a relatively conserved trait among L. paracasei strains. Indeed, a pilot study of a probiotic cocktail, VSL3, containing Lactobacillus sp. in MS patients has shown beneficial immunomodulatory effects [36]. We postulate that Lactobacillus is an important source of protective molecular patterns that are depleted in the context of myelin-specific autoimmunity. HK Lb interacts with other members of the gut microbiota to benefit neuroinflammation. HK Lb supplementation also restores systemic, low-level MAMP signaling; restricts chemokine production; and reduces infiltration of peripheral immune cells into the CNS. As we see prophylactic and therapeutic suppression of demyelinating disease using Lactobacillusassociated molecular patterns without the need for live bacteria, HK Lb warrants further investigation as a novel treatment for MS. JMSS, ABDPS, GB, AT, JEL, and RSF contributed to the analysis and interpretation of the data. JMSS, ABDPS, and JEL were involved in the manuscript drafting. JMSS, ABDPS, GB, RB, JEL, and RSF provided the critical review of the manuscript. All authors read and approved the final manuscript for publication.

Funding
This work was supported by the National Institute of Health 5R01NS065714 (R.S.F.), 1R01NS091939 (R.S.F.), and 5T32AI055434 (J.M.S.S.). The funding bodies played no role in the design of the study; in collection, analysis, or interpretation of the data; nor in the writing of the manuscript.

Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.