CSF GFAP levels in double seronegative neuromyelitis optica spectrum disorder: no evidence of astrocyte damage

Despite rigorous confirmation with reliable assays, some individuals showing the neuromyelitis optica spectrum disorder (NMOSD) phenotype remain negative for both aquaporin-4 (AQP4) and myelin oligodendrocyte glycoprotein (MOG) antibodies. We aimed to investigate whether double seronegative NMOSD (DN-NMOSD) and NMOSD with AQP4 antibody (AQP4–NMOSD) share the same pathophysiological basis, astrocytopathy, by measurement of cerebrospinal fluid (CSF) glial fibrillary acidic protein (GFAP) levels as a marker of astrocyte damage. Seventeen participants who (1) satisfied the 2015 diagnostic criteria for NMOSD, and (2) tested negative for AQP4 and MOG antibodies confirmed with repeated cell-based assays, and (3) had available CSF samples obtained at the point of clinical attacks, were enrolled from 4 medical centers (South Korea, Germany, Thailand, and Denmark). Thirty age-matched participants with AQP4–NMOSD, 17 participants with MOG antibody associated disease (MOGAD), and 15 participants with other neurological disorders (OND) were included as controls. The concentration of CSF GFAP was measured using enzyme-linked immunosorbent assay. CSF GFAP levels in the DN-NMOSD group were significantly lower than those in the AQP4–NMOSD group (median: 0.49 versus 102.9 ng/mL; p < 0.001), but similar to those in the OND (0.25 ng/mL) and MOGAD (0.39 ng/mL) control groups. The majority (90% (27/30)) of participants in the AQP4–NMOSD group showed significantly higher CSF GFAP levels than the highest level measured in the OND group, while no participant in the DN-NMOSD and MOGAD groups did. These results suggest that DN-NMOSD has a different underlying pathogenesis other than astrocytopathy, distinct from AQP4–NMOSD.


Introduction
Since the discovery of aquaporin-4 (AQP4) antibodies, neuromyelitis optica spectrum disorder (NMOSD) is defined as a neuro-inflammatory disease of the central nervous system (CNS), separate from multiple sclerosis [1]. The majority (up to 90%) of individuals with NMOSD defined by the 2015 revised diagnostic criteria Open Access *Correspondence: hojinkim@ncc.re.kr 1 Department of Neurology, Research Institute and Hospital of National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang, Korea Full list of author information is available at the end of the article show seropositivity for AQP4 antibody [2,3]. However, the criteria allow the diagnosis of NMOSD in individuals without AQP4 antibodies [4][5][6]. A subset of individuals with a NMOSD phenotype, but without AQP4 antibodies, test positive for the myelin oligodendrocyte glycoprotein (MOG) antibody, which primarily targets myelin antigens [7]. Although some clinical features overlap with AQP4 antibody positive NMOSD (AQP4-NMOSD), MOG antibody associated disease (MOGAD) has been recently defined as a distinctive disease entity, with elucidation of plausible different pathophysiology [8]. Despite repeated rigorous antibody measurements with reliable assays, some individuals with NMOSD phenotype remain persistently negative for both AQP4 and MOG antibodies [9][10][11][12]. Such double seronegative NMOSD (DN-NMOSD) poses diagnostic and therapeutic challenges in clinical practice and the classification of DN-NMOSD within the neuro-inflammatory diseases of the CNS remains unknown.
AQP4-NMOSD is a primary astrocytopathy mediated by antibodies selectively targeting the water channel protein AQP4, which is abundantly located on astrocytic foot processes in the CNS [1]. Glial fibrillary acidic protein (GFAP), a major constituent of the astrocyte cytoskeleton, can reflect astrocyte injury in AQP4-NMOSD when measured in body fluids, such as cerebrospinal fluid (CSF) [13][14][15]. This study aimed to investigate whether DN-NMOSD and AQP4-NMOSD share the same pathophysiological basis, astrocytopathy, by comparing the CSF GFAP levels at clinical exacerbation.

Methods
This international collaborative study enrolled participants from 4 medical centers (South Korea, Germany, Thailand, and Denmark). Participants who (1) satisfied the 2015 diagnostic criteria for NMOSD, [2] (2) were seronegative for both AQP4 and MOG antibodies following rigorous confirmation with reliable assays, and (3) had CSF samples available that were obtained at the point of clinical attack, were included. Seventeen CSF samples were obtained from individuals with DN-NMOSD (11 from South Korea, 2 from Germany, 1 from Thailand, and 3 from Denmark). Thirty age-matched participants with AQP4-NMOSD and 17 participants with MOGAD were included as controls. The MOGAD group included 10 patients who satisfied and 7 who did not satisfy the 2015 criteria for NMOSD. In addition, 15 age-matched controls with other neurological disorders (OND: 4 primary headache, 3 idiopathic sixth cranial neuropathies, 1 compressive myelopathy, 1 sub-acute combined degeneration, 1 hemi-facial spasm, 1 diabetic polyneuropathy, 1 brachial plexopathy, 1 non-specific white matter disease, 1 adrenoleukodystrophy, and 1 suspected motor neuron disease), not expected to present with underlying astrocytopathy, were included. All CSF samples were collected within 1 month of clinical exacerbation and most of the samples were obtained before the initiation of high dose steroid therapy (94% (16/17) in DN-NMOSD, 83% (25/30) in AQP4-NMOSD, and 71% (12/17) in MOGAD).
The serostatus of AQP4 antibodies was rigorously confirmed using two different methods: an in-house live cell-based assay (CBA) conducted at the National Cancer Center (NCC, South Korea) [16], and a commercial CBA (Euroimmun, Luebeck, Germany). The serostatus of anti-MOG antibodies was determined using the inhouse CBA at the NCC with live transfected cells with a full-length human MOG [17]. Additional file 1: Table S1 shows titers of AQP4 and MOG antibodies at the time of CSF sampling. Repeated examinations were performed during the course of the disease, at minimum of two different timepoints including at least one acute exacerbation in participants with DN-NMOSD. The seronegative status of the AQP4 and MOG antibodies in the sera, at the same timepoint with the CSF sample collection, 6 participants from Germany, Thailand, and Denmark was double-checked with CBA at each center and NCC. Antibody tests in CSF were also performed to eliminate the possibility of AQP4 and MOG antibodies detectable only in the CSF [18][19][20]. CSF GFAP concentrations were evaluated in duplicate by an independent investigator who was blinded to the diagnosis using an enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's protocol (BioVendor, Minneapolis, MN, USA).

Statistical analysis
GFAP levels across groups were compared by the Kruskal-Wallis test and post-hoc analysis was conducted using Dunn's multiple comparisons test. Table 1 shows the demographics of the participants. The mean age at sampling of the participants with DN-NMOSD, AQP4-NMOSD, MOGAD, and OND was 32.3 ± 9.6, 35.2 ± 11.1, 31.2 ± 7.3 and 36.8 ± 7.4 years, respectively. Table 2 demonstrates the clinical and paraclinical features of the participants with DN-NMOSD.
None of the DN-NMOSD and MOGAD groups showed higher CSF GFAP levels than the highest measured CSF GFAP level in participants with ONDs (primary headache, 1.48 ng/mL). In contrast, the CSF GFAP levels of most participants (27/30, 90%) in the AQP4-NMOSD group were higher than the highest measured CSF GFAP level in the OND group, and the remaining 3 participants with AQP4-NMOSD experienced relatively mild clinical symptoms (2 with optic neuritis and 1 with sensory-only partial myelitis).

Discussion
The CSF GFAP levels in the rigorously confirmed DN-NMOSD group were significantly lower than those in the AQP4-NMOSD group, but did not differ from those in the OND or MOGAD group. Consistent with previous results, [12][13][14][15] CSF GFAP levels in participants with AQP4-NMOSD were significantly higher than those in participants with ONDs or MOGAD. These findings suggest a discriminative underlying pathophysiology in DN-NMOSD other than astrocytopathy, distinguishing it from AQP4-NMOSD.
It is important to confirm the truly negative status of AQP4 and MOG antibodies to facilitate a fair comparison between DN-NMOSD and AQP4-NMOSD. Therefore, repeated tests using reliable CBAs were performed at least two different timepoints, particularly in the clinical exacerbation, to detect the positive conversion of AQP4 and MOG antibodies during subsequent clinical relapse [21]. At least two different assays with high sensitivity and specificity were used [16,17], in addition to CSF assays, because antibodies in some cases may be only detectable in CSF but not in serum [18][19][20]. A previous study reported that CSF GFAP levels in 2 of 3 participants with DN-NMOSD were elevated above those in non-neurological controls (highest level: 2.3 ng/mL) and increased over the median CSF GFAP level (5.40 ng/mL) of AQP4-NMOSD [12]; whereas no elevation in CSF GFAP levels was observed in 17 participants with DN-NMOSD relative to those in controls with ONDs (highest level: 1.48 ng/mL) in current study. The reason for this discrepancy is yet to be uncovered. Given the possible heterogeneity of DN-NMOSD, there would be a subset of patients with astrocytopathy due to yet unidentified autoantibodies or other causes in 3 DN-NMOSD patients of previous study, but not in 17 patients of current study. Alternatively, it might simply result from the difference in the confirmation of double negativity against AQP4 and MOG antibodies. The median CSF GFAP level of controls with ONDs (0.25 ng/mL) in the current study was comparable to that of previous studies (median 0.5 ng/ mL in non-neurological controls [12] and mean 0.6-0.7 ng/mL in controls with ONDs [13,14]).

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