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Table 2 Summary of antibodies used for immunohistochemistry

From: Dysregulation of the complement cascade in the hSOD1G93Atransgenic mouse model of amyotrophic lateral sclerosis

Antibody

Manufacturer

Dilution

In combination with

Rat anti-mouse C1q

Hycult Biotechnology1

1:1,000

GFAP, Iba-1 and ChAT

Rat anti-mouse C3b

Hycult Biotechnology1

1:50

GFAP, Iba-1 and ChAT

Mouse anti-mouse C5

Hycult Biotechnology1

1:1,000

GFAP, CD11b, ChAT

Rat anti-mouse CD88

AbD Serotec2

1:250

GFAP, Iba-1, ChAT

Mouse anti-mouse GFAP

BD Biosciences3

1:1,000

C1q, C3b, C5, CD88

Rabbit anti-mouse Iba-1

Wako4

1:400

C1q, C3b and CD88

Rat anti-mouse CD11b

Abcam5

1:300

C5

Goat anti-mouse ChAT

Chemicon6

1:100

C1q, C3b, C5, CD88

  1. GFAP, glial fibrillary acidic protein; ChAT, choline acetyltransferase; Iba-1, ionized calcium-binding adapter molecule-1;1Hycult Biotechnology, Netherlands; 2 AbD Serotec; 3 BD Biosciences, Sydney, NSW, Australia; 4 Wako, Osaka, Japan; 5 Abcam, Sydney, NSW, Australia; 6 Chemicon, Billerica, MA, USA.
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Journal of Neuroinflammation

ISSN: 1742-2094