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Figure 3 | Journal of Neuroinflammation

Figure 3

From: TSG (2,3,4’ ,5-tetrahydroxystilbene 2-O-β-D-glucoside) suppresses induction of pro-inflammatory factors by attenuating the binding activity of nuclear factor-κB in microglia

Figure 3

Role of TSG in injury of hippocampal neurons induced by BV-2 cell-derived conditioned medium. (A) Pretreatment with TSG (30 minutes, 50 μM) inhibited the increase in apoptotic nuclei in primary hippocampal neurons stimulated with BV-2 cell-derived conditioned medium. (B) Quantitative analysis of apoptotic nuclei percentage in neurons (n = 5, **P < 0.01 versus control; *P < 0.05 versus the lipopolysaccharide (LPS) alone-treated group). (C) Representative images showing that TSG pretreatment (50 μM, 30 minutes) reduced the cleavage of caspase-3 in primary hippocampal neurons stimulated with BV-2 cell-derived conditioned medium. (D) Quantitative analysis of cleaved caspase-3 in neurons (n = 3, *P < 0.05 versus control or the LPS alone-treated group). (E) Quantitative analysis of lactate dehydrogenase (LDH) content in LPS/TSG-treated hippocampal neurons (n = 6, **P < 0.01 versus control; *P < 0.05 versus the LPS alone-treated group). All data were shown as mean ± standard error. NS, no significance; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

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Journal of Neuroinflammation

ISSN: 1742-2094