Table 1 Oligonucleotide primers used for RT-PCR
Primer | Tm° | Sequence (5’-3’) |
|---|---|---|
HPRT for | 64.1 | TTGCTGGTGAAAAGGACCTC |
HPRT rev | 64.0 | TCCACTTTCGCTGATGACAC |
β-actin for | 64.2 | TTGCTGACAGGATGCAGAAG |
β-actin rev | 64.0 | TGATCCACATCTGCTGGAAG |
GAPDH for | 63.4 | CTCCCATTCTTCCACCTTTG |
GAPDH rev | 63.8 | ATGTAGGCCATGAGGTCCAC |
IL-1β for | 67.0 | TACCTATGTCTTGCCCGTGGAG |
IL-1β rev | 67.6 | ATCATCCCACGAGTCACAGAGG |
TNFα for | 64.0 | TGCCTCAGCCTCTTCTCATT |
TNFα rev | 63.2 | GGGCTTGTCACTCGAGTTTT |
IL-6 for | 62.5 | AAAGCCAGAGTCATTCAGAGC |
IL-6 rev | 63.8 | GAGCATTGGAAGTTGGGGTA |
IL-10 for | 64.0 | CCTGCTCTTACTGGCTGGAG |
IL-10 rev | 63.8 | TGTCCAGCTGGTCCTTCTTT |
IL-1R1 for | 63.7 | TTGTGTTTAAGCTGTTGCCG |
IL-1R1 rev | 62.4 | GTAACCTCGATGGTATCTTCCC |
IL-1R2 for | 61.9 | AGCACGTTTATCTCAGTGGC |
IL-1R2 rev | 65.5 | GTGACTGGATCAAAAATCAGCG |
TNFR1/p55 for | 61.9 | AGTCTACTGTGCCGATATCCC |
TNFR1/p55 rev | 64.9 | CTTTGACAGGAGCTGAATCCC |
TNFR2/p75 for | 64.0 | AAATGCAAGCACAGATGCAG |
TNFR2/p75 rev | 64.3 | CAGCAGACCCAGAGTTGTCA |
IL-6R for | 63.8 | CTGGTTCCTCTCCTCACCC |
IL-6R rev | 64.9 | TGTTGCTGTTGTCATTAGGGC |
IL-10R for | 63.8 | ATTTCACCGTGACCAACCTC |
IL-10R rev | 64.1 | CCAGGATGTGAATGTCATCG |