Figure 5

Inhibitory effect of HPB242 on nuclear factor-kappa B (NF-κB) translocation and DNA binding activity in the Tg2576 mouse brain. Effect of HPB242 on translocation of p50 in the Tg2576 mice brain; confocal microscopy images of the nuclear translocation of NF-kB-p50 were observed in microglia (A) and astrocytes (B) in the Tg2576 mouse brain. Effect of HPB242 on NF-κB activity in the Tg2576 mouse brain were determined by gel electromobility shift assay (EMSA) as described in Materials and Methods (C). Representative results were obtained from at least three different sets of experiment (n = 8). Inhibitory effect of thiacremonone on nuclear translocation of p50 and p65 and effect of HPB242 on p-IκB and IkB expression in the Tg2576 mice brain (D). Nuclear translocation of p50, p65, p-IκB and IκB expression levels in activity in the Tg2576 mice brain were determined by western blot (n = 8).