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Figure 8 | Journal of Neuroinflammation

Figure 8

From: Toll-like receptor 4 mediates microglial activation and production of inflammatory mediators in neonatal rat brain following hypoxia: role of TLR4 in hypoxic microglia

Figure 8

Suppression of Toll-like receptor 4 (TLR4) with siRNA reduced the production of reactive oxygen species (ROS) and nitric oxide (NO) induced by hypoxic stress in BV-2 cells. Intracellular ROS and production of NO in BV-2 cells transfected with control siRNA, control siRNA + hypoxia, transfected with TLR4 siRNA and TLR4 siRNA + hypoxia were checked. (A) The upper panel shows cell counts (y-axis) and log10 expression of fluorescence intensity (x-axis). The lower panel is a bar graph showing a significant change in the fluorescence intensity of intracellular ROS production following the above treatments. Note the ROS production, which is increased after hypoxic exposure in control siRNA-transfected BV-2 cells, is significantly decreased after hypoxic exposure in the TLR4-transfected group. (B) NO production in supernatant also shows a similar change as with ROS in the different groups mentioned above *P <0.05 and **P <0.01. The values represent the mean ± SD in triplicate.

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Journal of Neuroinflammation

ISSN: 1742-2094