Figure 4

Macrophages in the brain after repeated mild fluid percussion injury are reduced by the anti-CD11d treatment. (A) Photomicrographs of brain sections at the injury or equivalent site at 72 h after injury in the cortex in sham-injured, 1B7 control and anti-CD11d-treated rats immunostained by an anti-ED1 antibody. Format is similar to that described for Figure 3. Scale bars = 100 μm. (B) Macrophage protein, identified by western blotting in brain homogenates expressed as mean values (± standard error of the mean) with a representative autoradiogram shown above each set of histograms. Macrophage protein is shown at 72 h and 8 weeks in sham-injured, 1B7 and anti-CD11d groups (n = 6 all groups). A.U., arbitrary units; *different from sham group; #different from 1B7 group; all P ≤ 0.05 with the exception that gray # indicates P = 0.073.