Figure 1

Reduced T-cell reactivity in mice treated with Fn14·TRAIL during induction of experimental autoimmune encephalomyelitis (EAE). Mice were treated with 200 μg/day Fn14·TRAIL subcutaneously (s.c.) for 9 days. Pooled lymphocytes were obtained 9 days post-EAE induction. (A) Fn14·TRAIL reduced T cell proliferation induced by encephalitogenic Ag MOG_35-55. (B) Fn14·TRAIL reduced production of Th1 lineage cytokine IFN γ and Th17 lineage IL-17 with no significant change in Th2 cytokine IL-10. (C) Fn14·TRAIL effect on mRNA levels of Th2 lineage transcription factor GATA-3, Th17 RORγT and T-bet factors. (D) Splenocytes collected on day 9 were immune-stained and analyzed by fluorescence-activated cell sorting (FACS) for the percentage of CD4⁺CD25⁺FoxP3⁺ cells. (n = 5 in each group). *P <0.05, **P <0.01.