Figure 6
Potent inhibition of LPS-induced PGE2 (A and C) and 8-iso-PGF2α formation (B and D) by two highly selective COX-1 inhibitors (SC-560 and VAS). After 30 min pre-stimulation with the inhibitors, LPS (10 ng/mL) was added to the culture medium, and the amounts of PGE2 and 8-iso-PGF2α were determined using specific enzyme immunoassays after 24 h incubation with LPS. For all panels, histograms represent mean ± S.E.M. of 4 independent experiments. Statistical analysis was performed using one-way ANOVA followed by Student-Newman-Keuls post-hoc test. *p < 0.05, **p < 0.01 and ***p < 0.001 with respect to LPS control.