Figure 2

Expression of TLR11 in brains of mock infected control and NCC mice analyzed by immunofluorescence microscopy. Balb/c mice were infected intracranially with M. corti and sacrificed at various times p.i. Brain crysections were analyzed for expression of TLR11 and cell specific markers using fluorochrome conjugated antibodies. Nuclear staining DAPI is blue. (A) Mock infected control stained with TLR11 specific antibody (400×). (B) M. corti infection at 1 wk showing TLR11 positive staining in neurons (400×). (C) M. corti infection at 3 wk when maximum TLR11 expression was observed (400×). (D) M. corti infection at 6 wk showing TLR11 positive staining (400×). (E) Mock infected control mice stained with TLR11 and neuron specific antibody (Neun) (400×). The insert e' represents a 2× magnification of a selected area depicting TLR11 expression (Red) on neurons. (F) M. corti infection at 3 wk showing TLR11 positive neurons, merged image (yellow/orange; 400×). (G) Mock control mice stained with anti-TLR11 and anti-CD11b antibody (400×). (H) M. corti infection at 1 wk showing TLR11 and CD11b double positive cells, merged image (yellow/orange; 400×). Insert h' is a 2× magnification of a selected area to better illustrate TLR11 and CD11b double positive staining (I) The relative levels of TLR11 expression in mock and M. corti infected animals was calculated as described in experimental procedures.