Figure 4

NFκB activity in neurons cannot be evoked by supplementing key components of its pathway. IKK1, IKK2, IKK3, NIK, β-TrCP1, and TRAF2 were transfected either alone or in combination as indicated to test NFκB activity in neocortical neurons by reporter assays with a κB-driven firefly luciferase construct. Some transfection conditions were also further treated with rat TNFα (1–100 ng/ml). Rat RelA transfection served as a positive control. After 24 h, firefly luciferase activities were determined relative to Renilla luciferase reference. Values reflect the mean ± SEM of triplicate cultures. Except RelA transfection, no condition created a significant change compared to control.