Table 1 Primary antibody and antibody/fluorochrome-conjugates against various immune mediators, B. burgdorferi, and cell phenotypes
From: Possible role of glial cells in the onset and progression of Lyme neuroborreliosis
MEDIATOR/CELL TYPE | PRIMARY AB/SOURCE | ISOTYPE | DILUTION/CONCENTR. |
|---|---|---|---|
IL-6 | anti-human IL-6 (Abcam Inc. Cambridge, MA) | Rabbit IgG | 1:1000 |
IL-8 | anti-human IL-8 RDI Flanders, NJ | Rabbit IgG | 10 μg/ml |
CCL2 | anti-human CCL2 (BD) | Mouse IgG1 | 10 μg/ml |
| Â | anti-human CCL2 (Abcam) | Rabbit IgG | 1:10 |
CXCL13 | anti-human CXCL13 (R&D) | goat IgG | 5 μg/ml |
B. burgdorferi | anti-whole cell preparation (Accurate Chemicals, Westbury, NY) | Rabbit IgG | 1:250 |
| Â | anti- B. burgdorferi-FITC (Kirkegaard and Perry, Gaithersburg, MD) | Goat | 1:10 |
Astrocyte | anti-human GFAP-cy3 (Sigma St Louis, MO) | Mouse IgG1 | 1:200 |
| Â | anti-human GFAP (Sigma) | Mouse IgG1 | 1:200 |
Astrocyte, Oligodendrocyte, Schwann cell, satellite cell | anti-bovine S-100 (Sigma) | Rabbit IgG | 1:1000 |
Microglia | anti-Iba1 synthetic peptide (Wako Pure Chemicals, Richmond, VA) | Rabbit IgG | 1:100 |
Endothelial cells | anti-human GLUT-1 (Chemicon) | Rabbit IgG | 1:50 |
Neuron | anti-mouse NeuN (Chemicon) | Mouse IgG1 | 1:20 |
T cell | anti-human CD3 (Dako, Carpinteria, CA) | Mouse IgG2a | 1:10 |
B cell | anti-human CD20 (Dako) | Mouse IgG1 | 1:200 |
Macrophage | anti-human CD68 (Dako) | Mouse IgG1 | 1:50 |