Figure 3

Uptake and clearance of rat and human IgG and rat IgM by Purkinje cells over time. Uptake of rat IgG (Panels A, B), human IgG (Panels D, E), and rat IgM (Panels G, H) (red) is demonstrated in Purkinje cells studied at 6 hours (Panels A, D, G), with more extensive uptake visible at 24 hours (Panels B, E, H). In these figures Cy5 labels immunoglobulins red. IgG or IgM are no longer detectable in cultures studied after 24-48 hour incubation after transfer to media lacking the immunoglobulin (Panels C, F, I). Yellow fluorescence, as would be indicative of SYTOX green staining of dead cells containing IgG was not apparent. (Objective magnification 40×)