Cerebellar Purkinje cells incorporate immunoglobulins and immunotoxins in vitro: implications for human neurological disease and immunotherapeutics

Table 2 Interaction of GK1.5 IgG monoclonal, free daunorubicin, and GK1.5-daunorubicin immunotoxin with Purkinje cells

Reagent	Uptake by Purkinje cells	Purkinje cell death^a
GK1.5 monoclonal rat anti-CD4+ antibody	Identical to normal rat IgG (See Table 1)	<5% of cells^b
Unconjugated (free) daunorubicin	No detectable uptake	<5% of cells^b
GK1.5-daunorubicin immunotoxin	Initial uptake at 4 hours Widespread uptake by 6 hours	6 hours: <10% of cells 8 hours: 90% of cells

a. As indicated by intracellular presence of the cell death marker, SYTOX green in cells containing the IgG immunotoxin.
b. At 8 hours

ISSN: 1742-2094