Figure 1
From: Mechanisms of tumor necrosis factor-α-induced interleukin-6 synthesis in glioma cells
Effects of TNF-α on IκB phosphorylation and degradation and NFκB phosphorylation. Cultured cells were stimulated with 10 ng/ml TNF-α for the indicated period. Cell extracts were analyzed by Western blotting using antibodies against phospho-specific IκB, IκB, phospho-specific NFκB, NFκB or GAPDH. Similar results were obtained with two additional and different cell preparations.