Figure 3
From: Mechanisms of tumor necrosis factor-α-induced interleukin-6 synthesis in glioma cells
Effects of wedelolactone on TNF-α-induced phosphorylation of NFκB. Cultured cells were pretreated with various concentrations of wedelolactone or vehicle for 60 min, and then stimulated with 10 ng/ml TNF-α or vehicle for 10 min. Cell extracts were analyzed by Western blotting using antibodies against phospho-specific NFκB or NFκB. Similar results were obtained with two additional and different cell preparations.