Figure 6
From: Mechanisms of tumor necrosis factor-α-induced interleukin-6 synthesis in glioma cells
Effect of JAK inhibitor I on TNF-α-induced IL-6 release. (A) Cultured cells were pretreated with 10 nM JAK inhibitor I for 60 min, and then stimulated with 10 ng/ml TNF-α or vehicle for 36 h. Each value represents the mean ± SD of triplicate independent determinations of a representative experiment carried out three times. *P < 0.05 in comparison to the value of TNF-α alone. (B) Cultured cells were pretreated with various concentrations JAK inhibitor I or vehicle for 60 min, and then stimulated with 10 ng/ml TNF-α or vehicle for 150 min. Cell extracts were analyzed by Western blotting using antibodies against phospho-specific STAT3 or STAT3. Similar results were obtained with two additional and different cell preparations.