Figure 8
From: Mechanisms of tumor necrosis factor-α-induced interleukin-6 synthesis in glioma cells
Effect of apocynin on TNF-α-induced IL-6 mRNA expression. Cultured cells were pretreated with 0.1 mM apocynin or vehicle for 60 min, and then stimulated with 10 ng/ml TNF-α for 6 h. Total RNA was isolated and transcribed into cDNA. The expressions of IL-6 mRNA and GAPDH mRNA were quantified by real-time RT-PCR. IL-6 mRNA levels were normalized with those of GAPDH mRNA. Each value represents the mean ± SD of triplicate independent determinations of a representative experiment carried out three times. Similar results were obtained with two additional and different cell preparations. *P < 0.05 in comparison to the value of unstimulated cells. **P < 0.05 in comparison to the value of TNF-α alone.