Figure 9
From: Mechanisms of tumor necrosis factor-α-induced interleukin-6 synthesis in glioma cells
Effect of apocynin on TNF-α-induced the phosphorylation of IκB, NFκB, MAP kinases and STAT3. Cultured cells were pretreated with 0.1 mM apocynin for 60 min, and then stimulated with 10 ng/ml TNF-α for 10 min (A) or 150 min (B). Cell extracts were analyzed by Western blotting using antibodies against phospho-specific IκB, phospho-specific NFκB, phospho-specific p38 MAP kinase, phospho-specific SAPK/JNK, GAPDH, phospho-specific STAT3 or STAT3. Similar results were obtained with two additional and different cell preparations.