Figure 5

The expression of adhesion molecule transcripts is altered by α7 expression. Examination of infiltrating cells and resident cells after croton oil treatment for 6 hours reveals that certain molecules important to cell recruitment and localization to inflamed tissue are expressed. As shown using qPCR, in α7KO mice transcripts for ICAM1 are enhanced considerably in infiltrating cells and to a lesser extent in resident cells relative to controls (WT mice values normalized to 1 were used to determine fold increase observed in the KO). Itga3 transcripts are increased in α7KO infiltrating cells. In contrast, E-selectin transcripts are elevated in resident cells (ear skin depleted of infiltrating cells) and especially in the α7KO, but these transcripts were not detected in infiltrating cells. Error bars represent standard error of the mean; * p < 0.05; ** p < 0.01.